Generatio GmbH was formed as a result of the merger of CAG GmbH, Tübingen and Generatio Sol. GmbH, Heidelberg.
Together we offer a unique combination of the latest technologies in diagnostics and data processing with 20 years of experience in the implementation of DNA programmes.
generatio has extensive expertise in genetic diagnostics and research, focusing on individual and breed health. We support owners, breeders, breed clubs, and veterinarians in their goals to provide the best care for their animals and customers. In addition, generatio’s longterm relationships with major dog studbooks allows the individual genetic data of each animal to be uniquely positioned within that of the entire breed population over many generation. This capability is being expanded to also include other species in research and diagnostic programmes.
Our internet presence comprises 3 areas:
- Information: URL: www.generatio.de (this website)
- User platform 'Animal Trust Center' - URL: https://atc.generatio.de
- Webshop: - URL: https://shop.generatio.de
Technologies in our laboratory
DNA fragment length analysis
In this technique, specific sections of DNA are amplified by PCR and then separated according to size in a matrix.
An included length standard provides the reference values by which the length of the individual fragments can be determined.
The resolution accuracy depends on the separation method and is accurate to one base.
Areas of application
- Determination of the size of STR markers that are typed in the course of the creation of a DNA profile identity.
- Assessment of hereditary characteristics in which the alteration is based in the insertion or deletion of DNA segments.
DNA sequencing after Sanger
Sequencing of DNA segments according to Sanger is the standard procedure up to a length of approx. 1000 bases. Here, too, a fragment length determination of PCR fragments takes place. For each individual base in the section to be sequenced, there is a fragment of specific length; lined up, they result in their sequence.
Areas of application
Analysis of hereditary characteristics in which the change (mutation) affects individual bases (SNPs) or short sections (insertions/deletions).
Array typing
'Arrays' are densely packed specific DNA probes mounted on a carrier, each recognising a single specific site of DNA. Depending on the design, up to 700,000 individual probes can be housed in parallel in a single array.
Areas of application
- Parallel diagnostic of hereditary characteristics where the alteration (mutation) affects single bases (SNPs)
- GWAS (Genome wide association studies) Identification of SNPs correlated with specific traits
NGS - Next Generation Sequencing
NGS is defined as all procedures in which the entire DNA/RNA information of a sample can be collected in a single examination. Using NGS, the whole genome information of a human or animal is available within a few days.
For NGS applications, we work together with our shareholder CeGaT GmbH.
Areas of application
- Parallel diagnostics of known hereditary characteristics at the sequence level
- Identification of causal sequence changes at specific loci.