DO FRESH BLOOD SAMPLES HAVE TO BE EDTA STABILIZED?
Yes. EDTA stabilization prevents blood coagulation and thus guarantees the availability of a sufficient number of intact leukocytes required for DNA extraction. For optimal results, after blood has been extracted the sample tubes must be gently shaken several times.
CAN EDTA STABILIZED BLOOD BE STORED IN ORDER TO COMBINE SAMPLES FOR SHIPMENTS?
It depends on the tests to be carried out with the DNA sample material. For simple genetic identity and parentage diagnosis EDTA stabilized blood samples (as long as they are well-blended) can be stored and shipped, both at room temperature, for several days.
WHAT IS THE MINIMUM AMOUNT OF BLOOD REQUIRED FOR A SUBMISSION?
This depends on the planned tests and purpose of storage. For diagnosis only without storage a few blood drops suffice. For diagnosis including storage we recommend a minimum of 2 ml fresh blood.
WHY ARE THERE DIFFERENT MARKER SETS PROPOSED FOR A SINGLE SPECIES?
DNA-based identity and parentage determination has been established since the mid 90's. At first, only (highly reputed) scientific laboratories carried out DNA tests. These labs put together their individual marker sets based on experience and availability. Later, a variety of commercial kits - sorts of prefabricated analysis building blocks - came to market. These kits contained different combinations of markers, and many additional labs could now offer DNA services. For the last several years, ISAG has been working to develop a standard marker panel for economic important species.
THERE IS A VARIETY OF MARKER SETS FOR DOGS. WHICH ONE IS THE CORRECT SET FOR MY ANIMAL?
Generatio offers DNA services for many years. Long-term DNA programs serviced by Generatio are always based on an identical working method. We guarantee this approach, for instance, for the Generatio marker set that was especially designed to deliver the most detailed information in respect to parentage. Comparatively, marker sets currently promoted by both ISAG and NIST are relatively "young" and don't guarantee long-term consistency of initially established characteristics in profiled animals. However, this feature is the most relevant aspect in long-term and thus economical DNA programs.
CAN DNA PROFILES THAT HAVE BEEN GENERATED BY DIFFERENT LABORATORIES BE COMPARED?
Generating DNA profiles may be standardized to the extent that comparing established identities is possible because profiles must be 100% identical. Combining profiles of different origin for the purpose of parentage evaluation requires utmost care. For example, because of naturally occurring mutations DNA profiles of certain descendants can display inconsistencies when compared with the actual genetic characteristics in the declared parent animals. These inconsistencies must be differenciated from those that clearly point to incorrect parentage.